However, although annotations continue to improve, inaccuracies are introduced by the need to computationally reconstruct full-length transcript isoforms from short-read data. Many projects have produced, or are aiming to produce, a reference transcriptome to synthesise the wealth of (highly redundant) sequencing information, although the resulting annotations vary due to differences in algorithms applied and the extent to which annotations are manually curated. We highlight three areas that pose potential barriers to effective information transfer and offer suggestions as to how these may be addressed: firstly, a lack of clarity about the strength of the evidence for individual transcripts in current annotations secondly, limitations to the transfer of information between nucleotide and protein databases thirdly, challenges relating to the nomenclature used for transcriptional events and RNA modifications, both for genomic researchers and the wider scientific community. Here, we outline how the status quo can result in information becoming siloed and/or ambiguous, using the CACNA1C gene, which encodes a voltage-gated calcium channel, as an example. However, there are multiple barriers to their efficient dissemination and their translation into functional insights. It is important that these findings are readily accessible to the wider scientific community to maximise their impact. The advent of cost-effective high-throughput nucleotide sequencing means that information about the transcriptome is accruing at an exponential rate, rapidly refining our understanding of the diversity of gene products.
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